Lumpy Skin Disease

 


LUMPY SKIN DISEASE (LSD)

·       It’s acute, sub-acute or chronic viral disease of cattle Caused by capripox virus and which is characterized by fever, Oedema of skin, Formation of nodules on skin and mucous membrane of internal organs, enlargement of lymph nodes and increased nasal discharge.

Synonyms:

·         Pseudourticaria

·         Neethling virus disease 

·         Exanthema nodularis bovis

It’s acute, sub-acute or chronic viral disease of cattle which is characterized by

ü  Fever

ü  Large lymph nodes

ü  Increased nasal discharge

ü  Oedema of skin

ü  Formation of nodules on skin and mucous membrane of internal organs



DISTRIBUTION

·         LUMPY SKIN DISEASE (LSD) now endemic in Southern Africa, Europe and Asian countries.

·         It’s sporadic in India and Bangladesh.

·         Lsd outbreaks among cattles was reported in odisha in india

ETIOLOGY

·         Family: Poxviridae

·         Caused by capripox virus of

·         It’s double stranded DNA virus. 

·         Enveloped virus having brick shape and it replicate in cytoplasm.

HOST

·         Cattle, Buffalo, yak.

·         RESERVOIR HOST: Giraffe, Impala, Arabian Oryx, gazelle.




TRANSMISSION

·         Mosquitoes

·         Ticks

·         Infection via skin lesion

·         Through infected bull semen  

·         Other secretions and Iatrogenic.



PATHOGENESIS

Entry of virus through skin or respiratory tract

Local virus replicate in epidermis , dermis (keratocytes) , or in respiratory epithelium

Virus enter in local lymph node and also in reticulo-endothelial cells

Virus got identified by macrophages and replicate in cytoplasm of macrophages

Virus exits via efferent lymphatic vessels into blood.

Virus disseminate Into the body and Enter skin via infected macrophages.

Replicate in endothelial cells.

Infected cells release cytokines and cytokine induced vasodialation.

Increased vascular permeability, leukocyte chemotaxis cause oedema and local inflammation

Langerhan cells close contact with endothelial cells in malphigian layer of skin.

Virus infect Langerhan cells and spread to endothelial cells of stratum basale and spinosum.

All these cells allow virus to replicate in them.

Stratum basale and spinosum gets necrosed and forms exudate filled nodules with debris cells.

Through adaptive immune response the the virus infection is resolved and the nodule gets healed and forms a scar.


CLINICAL SIGNS 

            ·         Invasive form :- fever (105-106°F)

§  Enlarged lymph nodes

§  Conjunctivitis

§  Lachrymation and hyper salivation

§  Nasal discharge ( thick off white mucoprulent)

 

    §  Eruption form:- Firm,

§  Round, painless and circumscribed nodule.

§  Oedema in legs, loins, dewlap and skin

 

    §  Necrosis form:- deep wound with a “punched out” appearance

§  “inverted conical zone" of necrosis or “sit fast necrosis”


    §  Mild form:- enlarge lymph node nodules over entire body healing

§  Within 3 to 6 weeks.


    §  Severe form:- difficult and noisy breathing due to nodules

§  Oedema in phyrnx and larynx.

§  Cessation of rumination

§  Nodules on oesophagus

§  Death due to asphyxia

 





     GROSS PATHOLOGY

ü  Pox lesion mucous membrane pharynx, trachea and udder.

ü  Yellow-red tinged oedematous fluid in s/c

ü  Nodules involving all layers of skin, digestive and respiratory systems

ü  Nodules and hemorrhages in facia over limb muscle and s/c    tissue

ü  Nasal mucus and oropharynx is ring appearance peculiar due to separation of necrotic epithelium to healthy tissue

 

     MICROSCOPIC PATHOLOGY

ü  Acanthosis with orthokertatotic or hyperkeratosis

ü  Hydronic degeneration (ballooning) of epidermal cells

ü  Intracytoplasmic eosinophilic viral inclusion bodies

ü  In superficial And deep dermis is moderate to severe perivascular infiltration

     SEQUALE

ü  Mastitis due to secondary bacterial infection

ü  Lamness due to inflammation and necrosis of joint, tendon

ü  Pneumonia

ü  Abortion, intrauterine infection in cow

     DIAGNOSIS

      ·         Field presumptive diagnosis (clinical signs and PM lesion)

      ·         Generalized skin multiple nodules

      ·         Inverted conical necrosis of skin nodules

      ·         Persistent fever with low mortality

 

   Laboratory diagnosis

·         Virus Isolation on cell culture -egg Inoculation

·         Electron microscopy

·         FAT, AGID, ELISA

·         PCR

·         Virus neutralization

·         Histopathology

·         Immunohistochemistry

      DIFFRENTIAL DIAGNOSIS

·         Peudo lumpy skin disease:No lymph node hypertrophy, Flat nodules a depression in centre, Affect only epidermis

·         Leucosis: variable  size nodules, non contagious

·         Dermatopholosis: Superficial papular lesion with scab & without lymph node enlargement

·         Tuberculosis: Subcutaneous persisting nodules,hypertrophy of lymph nodes

·         Demodex: Pustular nodules,hyperkeratosis of skin & scabs

·         Onchocercosis: Nodules localised on joints & tendons

·         Parafilariosis: Cutaneous nodules with haemorrhage

·         Urticaria: No general symptoms, Oedematous plaques & keratosis

·         Insect bites: Painful nodules, without necrotic zone

 TREATMENT 

      · Symptomatic and preventing secondary bacterial infection, combination of antimicrobials, anti-inflammatory, supportive therapy and anti-septic solutions.

      ·Treatment is costly as well as does not ensure full recovery therefore prevention is more beneficial to avoid economic losses due to hide damages , loss of milk due to mastitis and loss of animal product due to abortion,  myriads and death.

Homeo drugs for Lumpy Skin Disease

      · Variolinum 200 (Used: treat small pox like lesions and present catarrhal and gastric symptoms.)

      · Dulcamara 200(Used: itchy skin, boils, acne, warts, eczema)

      · Acid nitricum 200

      ·  Oscimum sanctum 30 and

      · Thuja 30

      ·   All above drugs once a day for 5 days

       · Calcaria flour 200(used: acne, arthritis,night terrors in children,ringworm on the scalp and vaginal discharges in women,) and treated Six animals All animals responded with in 3 to 5 days. No drop in milk yield.

     Vaccination: 

·         Attenuated LSDV vaccine

·         Attenuated SPPV vaccine

·         Attenuated Gorgan GTPV vaccine

 

Prevention & Control : 

üIsolation of infected animal

üAnimal suspected of febrile nodular skin disease should not be introduced in other animal
üReduce vector population in affected area
üUse of repellent on unaffected animals to minimize mechanical transmission of LDS
üEnsure strict control of animal movement from affected area
üAll biosecurity & strict sanitary measures should be applied
üSemen should not be collected from affected animals
üAwareness campaign

Special Thanks: 

Dr. Sneha modh

Dr. Gurjot singh

Dr. Manohar Upadhaya

Dr. Jibachha Shah 


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